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Document: Dialysis buffer for IgG isolation | Last modified: June 04, 2005
Dialysis Buffer For Isolation Of IgG From Egg Yolks

Need:
20 mM Trizma base ph=8
50 mM NaCl
0.1 M EDTA
10% Glycerol
0.02% NaAz
pH= 7.0

Stock:
0.1 M Trizma base pH=8
0.15 M NaCl
0.5 M EDTA
100% Glycerol
20% NaAz

Use:
200 ml of 0.1 M Tris
333 ml of 0.15 M NaCl
200 ml of 0.5 M EDTA
100 ml of100% Glycer.
1 ml of 20% NaAz

1. Measure out 200 ml of 0.1 M Tris and pour it into a 1 L beaker.

2. Measure out 333 ml of 0.15 M NaCl and then add it to the Trizma base.

3. Measure out 200 ml of 0.5 M EDTA and add it to the 1 L beaker.

4. Measure out 100 ml of 100% Glycerol and add it to the 1 L beaker.

5. Measure out 1 mL of 20% Na Az using a pipet and add to the 1 L beaker.

6. Measure the pH and adjust it to pH= 7.0.

7. Pour the solution into a 1 L graduated cylinder and adjust the volume to 1 L by adding DDW.

8. Place a stir bar in the solution and store it in the cold room on a stir bench.
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